Breakthrough in Neuroimaging Technology for Freely-behaving Rodents

Biophotonics Imaging Technology (BIT) Lab, led by Prof. Xingde Li at the Department of Biomedical Engineering, and lead author Dr. Ang Li, published a paper in Optica that reported their recent breakthrough in neuroimaging technology based on a scanning fiberscope: the implementation of a compact and lightweight fiberscope enabling dynamic two-photon neuroimaging in freely rotating and walking rodents.

In vivo imaging results demonstrated that the 2P fiberscope reliably recorded calcium dynamics of more than 50 neurons simultaneously in the motor cortices of freely-moving mice. The technology equipped active rotational tracking and compensation capabilities through an optoelectrical commutator (OEC) that allowed the animal to rotate and walk in arbitrary patterns during 2P imaging of neural activities. In addition, the new technology was equipped with custom dispersion management for femtosecond excitation laser, which improved neuroimaging SNR by more than 2X. 

Share this page onFacebookTwitter